Research Technologist
Baltimore, MD
Job Description

General Summary/Purpose:

The department of Biology is seeking a Research Technologist to work in a molecular biology and genetics laboratory on the Homewood Campus at the Johns Hopkins University starting September 2019. The Lab will work on deciphering the mechanistic principles of mRNA localization in Drosophila using single-molecule imaging techniques, genetics, in vitro reconstitutions and biochemistry. The goal of this research is to better understand how mRNAs enrich to subcellular organelles and how this enrichment drives cell growth, differentiation and organismal development.

The research technician will work closely under the supervision of the principle investigator to perform a variety of research activities, including imaging using various modalities (fixed and live single-molecule, super-resolution imaging and confocal microscopy), molecular biology, genetics, biochemistry, immunohistochemistry, and data analysis.

Specific Duties & Responsibilities:

  • Will create recombinant DNA using Molecular biology tools such as PCR, DNA and RNA purifications and cloning.
  • Will use Cell Biology approaches to grow and maintain mammalian and Drosophila cell cultures to perform measurements of mRNA enrichment in fixed and live cells.
  • Will perform fly husbandry to raise and handle Drosophila genetic crosses and maintain fly stocks.
  • Will use quantitative microscopy techniques, image analysis and general statistical analysis to investigate how mRNA distribute in Drosophila cells and embryos.
  • Will use immunohistochemistry to investigate the spatial distribution of proteins that drive mRNA enrichment in Drosophila cell lines and embryos.
  • Will participate in laboratory meetings with the opportunity to present work.
  • Will read literature related to scientific field.

Minimum Qualifications (Mandatory):

  • Bachelor's degree in Biology, Biochemistry, Bioengineering, or related field

Preferred Qualifications:

  • At least one year of undergraduate research experience is preferred.
  • Experience with molecular biology techniques (Cloning, PCR) and cell culture.

Special Knowledge, Skills, and Abilities:

  • Must be driven, organized, and actively seek new skills
  • Must be able to work independently
  • The research technician will have the opportunity to take on independent research projects including the development of new techniques, procedures, and tools.

This is an ideal opportunity for recent graduates to garner additional research experience of 2+ years before attending graduate or medical school. The position is also open to individuals with more extensive experience who are interested in broadening their skills.

Classified Title: Research Technologist
Role/Level/Range: ACRO37.5/02/CC
Starting Salary Range: $14.00 to $19.25 per hour
Employee group: Full Time
Schedule: M-F, 8:30 am - 5:00 pm
Exempt Status: Non-Exempt
Location: 01-MD:Homewood Campus
Department name: 10001352-Biology
Personnel area: School of Arts & Sciences

The successful candidate(s) for this position will be subject to a pre-employment background check.

If you are interested in applying for employment with The Johns Hopkins University and require special assistance or accommodation during any part of the pre-employment process, please contact the HR Business Services Office at For TTY users, call via Maryland Relay or dial 711.

The following additional provisions may apply depending on which campus you will work. Your recruiter will advise accordingly.

During the Influenza ("the flu") season, as a condition of employment, The Johns Hopkins Institutions require all employees who provide ongoing services to patients or work in patient care or clinical care areas to have an annual influenza vaccination or possess an approved medical or religious exception. Failure to meet this requirement may result in termination of employment.

The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.

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Job Type Full-time